Sri Nityananda Trayodashi is the auspicious appearance day of Sri Nityananda Prabhu. The Supreme Lord Sri Krishna appeared in Navadvipa as Sri Chaitanya Mahaprabhu to establish the Sankirtana Movement (the Yuga Dharma for this age of quarrel and hypocrisy). To help the Supreme Lord in His mission, Lord Balarama appeared as Nityananda Prabhu. He assisted Sri Chaitanya Mahaprabhu by spreading the holy name of the Lord throughout Bengal.

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Nityananda Prabhu appeared in the village of Ekachakra, in the district of Birbhum (West Bengal) as the son of Padmavati and Hadai Pandita in the year 1474 AD. He was born on the thirteenth day of the bright fortnight in the month of Magha (Magha Shukla Trayodashi).On the day of Nityananda Trayodashi, devotees fast till noon and pray to Lord Nityananda for spiritual strength. Agrand abhisheka is performed to Sri Sri Nitai Gauranga. The Deities are bathed with scented water and given a sandal oil massage.

After the massage the Deities are bathed with panchamrita (milk, curd, sweetened water, ghee and honey) and panchagavya.The Deities are then bathed with exotic fruit juices and water mixed with herbal extracts. The sarvaushadhi snana is followed by 108 kalasha snana and concludes with pushpa-vrishti (showering of fragrant flowers on the Deities). The Deities are offered a feast of 56 items (chappan bhog). The Shayana Arati concludes the festival.